MVDr. Jiří Šimůnek, CSc.

Education

1989	Ph.D. Thesis: Manipulation of the Rumen Fermentation.
1976-1982	Veterinary and Pharmaceutical University Brno

 

Professional experience

2000-now	Independent research worker, Institute of Animal Physiology and Genetics, AS CR, v.v.i., Prague
1989-2000	Research worker, Institute of Animal Physiology and Genetics, AS CR, v.v.i., Prague
1983-1989	Specialist, Institute of Animal Physiology and Genetics, AS CR, v.v.i., Prague

 

Major scientific interests

• Microbiology and biochemistry of anaerobic bacteria from the animal and human digestive tract
• chitin enzyme degradation
• chitinases
• chitinolytic bacteria of the human and animal gastrointestinal tract

 

Fellowships, prizes

2011	3rd Award Prize for poster presentation, conference EUCHIS 2011, St. Petersburg
2009	3rd Award Prize for poster presentation, conference EUCHIS 2009, Venice

 

Research fellowships and study visits

1995-1996	The Kielanowski Institute of Animal Physiology and Nutrition, Jablona, Poland
1989	Rowett Research Institute, Aberdeen, Scotland, U.K.
1988	Institute of Animal Physiology, Alma-Ata, Kazakhstan
1987	Institute of Animal Physiology and biochemistry, Lviv, Ukraine

 

Publications

2011	Tishchenko G., Šimůnek J., Bartoňová H., Dušková J., Dohnálek J.: Sample preparation in separation of the extracellular chitinolytic enzymes of the human intestinal bacterium Clostridium paraputrificum J4 from the culture fluids. J. Chromatography B, 879: 2175-2178
	Tishchenko G., Šimůnek J., Brus J., Netopilík M., Pekárek M., Walterová Z., Koppová I., Lenfeld J.: Low-molecular-weight-chitosans: Preparation and characterization. Carbohydrate Polymers, 86:1077-1081
	Dušková J., Tishchenko G., Ponomareva E., Šimůnek J., Koppová I., Skálová T., Šťěpánková A., Hašek J., Dohnálek J.: Chitinolytic enzymes from bacterium inhabiting human gastrointestinal tract – critical parameters of protein isolation from anaerobic culture. Acta Biochima Polonica 58:261-263
2010	Mrázek J., Koppová I., Kopečný J., Šimůnek J., Fliegerová K: PCR-DGGE based study of fecal microbial stability during the long-term chitosan supplementation of human. Folia Microbiologica 55:352-358
	Tishchenko G., Koppová I., Šimůnek J., Dohnálek J.: Extracellular comlex of chitinolytic enzymes of Clostridium paraputrificum strain J4 separated by membrane ultrafiltration, Folia Microbiologica 55:386-389
	Šimůnek J., Koppová I., Lukáš F., Tishchenko G., Belzecki G.: The antimicrobial action of low-molar-mass chitosan, chitosan derivatives and chitooligosaccharides on bifidobacteria. Folia Microbiol. 55: 379-382
	Tishchenko G., Koppová I.: Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan, Folia Microbiologica 53:249-254
	Lukáš F., Šimůnek J., Mrázek J., Kopečný J.: PCR-DGGE Analysis of bacterial population attached to the bovine rumen wall. Folia Microbiol. 55: 345-348
2008	Šimůnek J., Tishchenko G., Koppová I.: Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan, Folia Microbiologica 53:249-254
	Belzecki G., Miltko R., Michalowski T., Šimůnek J., Kopečný J.: chitinolytic aktivity of the sheep rumen ciliate Diploplastron affine. Folia Microbiol. 53: 201-203
2007	Kopečný J., Šimůnek J.: Chitosan application in food preparation and digestion. Adv. Chitin Sci. X, Senel S., Varum KM, Sumnu MM, Hincal AA, Eds., alp Offset, Ankara, pp. 467-473