Education

1996-1999	Ph.D. student at Institute of Freshwater Ecology and Inland Fisheries, Berlin Department of Biology and Ecology of Fishes Thesis: "The autecology of spined loach, Cobitis taenia" with special emphasis on the reproductive biology and ecology of early stages
1993-1994	Master student at Max-Planck-Institute of Limnology, Plön, Germany Thesis: "in-situ grazing rates of Daphnia during spring in Lake Schöhsee near Plön"
1989-1994	Student of Biology, Carl-von-Ossietzky University Oldenburg, Germany

Professional experience

1999-now

Researcher; Laboratory of Fish Genetics, IAPG AS CR, v.v.i., Liběchov, Czech Republic

Major scientific interests

My primary education is that of a zoologist and ecologist. After a brief period in limnology I worked on the autecology of various freshwater fishes, using experimental setups in microcosms in combination with field data from natural populations; the model organisms were mainly spined loaches. Studies on the spined loach (Cobitis taenia) revealed a large, but hidden diversity under this name, including the widespread presence of unisexually reproducing lineages of hybrid origin. Starting with analyses of the spined loaches in Germany and the Czech Republic via the diversity of Cobitis in Europe towards the genera and families of loach fishes worldwide, my work focused more and more on the diversity and evolutionary history of freshwater fishes in Europe and Asia. The analyses combine phylogenies that were reconstructed on the base of molecular genetic data (by colleagues with training in genetic techniques) with cytogenetical, morphological and geological data, in order to identify the impact of separation events, range extensions, cases of hybridisation and of polyploidisation as well as the climatic, tectonic and geographical changes in Eurasia during Cenocoic. At the end it is possible to reconstruct the whole evolutionary history of the analysed fishes and to conclude on the relative importance of the different evolutionary mechanisms.

Currently, together with other colleagues from the Laboratory of Fish Genetics we focus on the investigation of origin and stabilisation of a polyploid lineage within the family Botiidae (Cobitoidea) that appeared about 20 mya. Due to the complexity of the topic (particularly the high age of polyploid lineage), we apply a multi-method approach including analyses of DNA variability (sequencing, microsatellites), methods of classical as well as molecular cytogenetis, and transcriptomics.

Fellowships, prizes

2012	Award of CAS; member of a team of authors; topic: „Clonal vertebrates: Discovery, mechanisms, biodiversity and reconstruction with use of a model of loach fishes“

Membership in academic councils and editorial boards

European Ichthyological Society – member of board
Gesellschaft für Ichthyologie
Fisheries Society of the British Isles
Ichthyological Society of Japan

Research fellowships and study visits

2006	Museum of Natural History, London, United Kingdom
2006	Raffles Museum of Biodiversity Research, National University, Singapore
2004, 2005	Institut für Evolutionsbiologie, Universität Bonn, Bonn, Germany
2002, 2005	Museo National de Ciences Naturales, Department Biodiversidad y Biología Evolutiva, Madrid, Spain

Publications

10 selected publications (out of ~100 publications):

2014	Geiger M.F., M. T. Monaghan, V. Almada, R. Barbieri, M. Bariche, P. Berrebi, J. Bohlen, M. Casal-Lopez, G. B. Delmastro, G. P. J. Denys, A. Dettai, I. Doadrio, E. Kalogianni, H. Kärst, M. Kottelat, M. Kovačić, M. Laporte, M. Lorenzoni, Z. Marčić, M. Özulu?, A. Perdices, S. Perea, H. Persat, S. Porcelotti, C. Puzzi, J. Robalo, R. Šanda, M. Schneider, V. Šlechtová jr., M. Stoumboudi, S. Walter, J. Freyhof 2014. Spatial Heterogeneity in the Mediterranean Biodiversity Hotspot Affects Barcoding Accuracy of its Freshwater Fishes. Molecular Ecology Resources 14: 1210-1221. IF 2013: 5.626
2013	Bohlen, J., V. Šlechtová jr. 2013. Two new species of Schistura (Nemacheilidae) from Myanmar. Ichthyological Exploration of Freshwaters 24: 21-30. IF 2013: 2.275
	Symonová, R., Majtánová, Z., Sember, A., Staaks, G.B.O., Bohlen, J., Freyhof, J., Rábová, M. and Ráb, P. 2013. Genome differentiation in a species pair of coregonine fishes: an extremely rapid speciation driven by stress-activated retrotransposons mediating extensive ribosomal DNA multiplications. BMC Evolutionary Biology 13, 42. IF 2013: 3.407
2011	Bohlen, J., V. Šlechtová jr., H.H. Tan, R. Britz 2011: Molecular phylogeny of the freshwater fish genus Pangio (Cobitidae: Teleostei): Molecular Phylogenetics and Evolution 61: 854-865. IF 2010: 3.889 doi: 10.1016/j.ympev.2011.08.003
2008	Šlechtová, V. jr., J. Bohlen, A. Perdices (2008): Molecular phylogeny of the freshwater fish family Cobitidae (Cypriniformes: Teleostei): delimitation of genera, mitochondrial introgression and evolution of sexual dimorphism. Molecular Phylogenetics and Evolution 47: 812-831. IF 2008: 3.871 doi: 10.1016/j.ympev.2007.12.018
	Perdices, A., Bohlen, J. & I. Doadrio (2008): The molecular diversity of Adriatic spined loaches (Teleostei, Cobitidae). Molecular Phylogenetics and Evolution 46: 382-390. IF (2008): 3.871 doi: 10.1016/j.ympev.2007.05.007
2006	Bohlen, J., Šlechtová, V.jr., Bogutskaya, N. & J. Freyhof (2006): Across Siberia and over Europe: Phylogenetic relationships of the freshwater fish genus Rhodeus in Europe and the phylogenetic position of R. sericeus from the River Amur. Molecular Phylogenetics and Evolution 40: 856-865. IF 2006: 3.528 doi:10.1016/j.ympev.2006.04.020
	Bohlen, J., Perdices, A., Doadrio, I. & P. Economides (2006): Vicariance, colonisation and fast local speciation in Asia Minor and the Balkan as revealed from the phylogeny of spined loaches (Osteichthyes; Cobitidae). Molecular Phylogenetics and Evolution 39: 552-561. IF 2006: 3.528 doi: 10.1016/j.ympev.2005.12.007
	Šlechtová, V. jr., J. Bohlen, J. Freyhof & P. Ráb (2006): Molecular phylogeny of the Southeast Asian freshwater fish family Botiidae (Teleostei: Cobitoidea) and the origin of polyploidy in their evolution. Molecular Phylogenetics and Evolution 39: 529-541. IF 2006: 3.528 doi: 10.1016/j.ympev.2005.09.018
2004	Šlechtová, V. jr., J. Bohlen, J. Freyhof, H. Persat, G.B. Delmastro 2004: The Alps as barrier to dispersal in cold-adapted freshwater fishes? Phylogeographic history and taxonomic status of the bullhead in the Adriatic freshwater drainage. Molecular Phylogenetics and Evolution 33: 225-239. IF 2004: 4.213 doi:10.1016/j.ympev.2004.05.005