Ing. Jan Kopečný, DrSc.


1985-1997 DSc., Microbiology, Institute of Animal Physiology and Genetics.
Microbial Feed degradation in the Rumen.
1977-1981 CSc., Biochemistry, (eq. Ph.D.), Czechoslovak Academy of Sciences
In vitro incubation of rumen microbes.
supervisor: Ing. Stanislav Bartoš, DrSc.
1970-1975 Dipl. Ing., Biochemistry, Institute of Chemical Technology, Prague
Stability of lysosomes in meat post-mortem.
supervisor: prof. Ing. Jan Káš, DrSc.


Professional experience

2017-now Deputy director for Science
2007-2016 Director of IAPG CAS, v. v. i.
2003 Head of Laboratory of Anaerobic Microbiology
1991 Senior Scientific Officer


Major scientific interests

    • Anaerobic Microbiology

My studies started with rumen proteolytic, amylolytic and cellulolytic microbes and their hydrolytic enzyme production. Following years I was dealing with rumen microbes adhesion to feed particles. We introduced methods for anaerobic microbiology and developed probiotics for in vivo testing. Then we moved to biochemistry of rumen bacterium Butyrivibrio fibrisolvens and rumen fungi and interactions of rumen microbes in vitro and in vivo. It led to the development of enzymatic method for estimation of protein degradation in the rumen and feed digestibility for ruminants. I took a part in transformation of anaerobes. I was able with a shuttle vector with fluoroacetate dehalogenase to transform a many Pseudobutyrivibrio isolates. Transformation of undescribed isolates directed me to taxonomy of butyrate-producing bacteria and description of two new species: Butyrivibrio hungatei, Pseudobutyrivibrio xylanivorans. I was involved in description of Butyrivibrio proteoclasticus, later on.

Knowledge of butyrate-producing bacteria helped me to map changed microbiome in patients with the human bowel diseases: we described the effect of Inflammation Bowel Disease and Celiac Disease and we able to show the importance of probiotic treatment. It was possible to describe Bifidobacteria gut colonization in newborns, calves as well as in social insect digestive tract.

In last decade, we have detected presence of bifidobacteria in most vertebrates and many insect species. In frame of this, we described new bifidobacteria in the gut of bumblebees: Bifidobacterium bombi, B. bohemicum and B. actinicoloniformis and a new genus Bombiscardovia (Bombiscardovia coagulans).


Membership in academic councils and editorial boards

2012 Journal of Nutritional Ecology and Food Research, Editorial Board
2010 Folia Microbiologica, Editorial Board
2009 Bioprospect, Editorial Board
2009 Czech Society for Immunology
1999 Biotechnology Society of CR
1985 Czechoslovak Society for Microbiology
1983 Czech Society for biochemistry and Molecular biology
1981-2003 American Society for Microbiology


2016-2018 Kopečný J.
Structure and synergism of fibrolytic enzymes in the rumen.
Czech Science Foundation, 16-12431S
2015-2019 Němečková Irena, MILCOM, Kopečný J.
Fermented dairy products and cheeses for healthy human nutrition, technological procedures of their production and evaluation methods accenting high level of microbiological safety and improved nutritional parameters.
NAZV QJ1510338
2012-2015 Wallace R. John, Rowett (UK), Kopečný J.
Ruminomics: Connecting the animal genome, gastrointestinal microbiomes and nutrition to improve digestion efficiency and the environmental impacts of ruminant livestock production.
FP7 289319
2011-2014 Tlaskalová Helena, MBÚ, Kopečný J.
Bacteria in etiology, prevention and therapy of experimentally induced intestinal inflammation and colorectal carcinoma.
GA ČR 304/11/1252.



2016 Grilli D.J., Fliegerová K., Kopečný J., Lamac S.P., Egea V., Sohaefer N., Pereyra C., Ruiz M. S., Sosa M.A., Arenas G.N., Mrázek J.
Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet.
Anaerobe, 42, 17 - 26
  Grilli D.J., Mrázek J., Fliegerová K., Kopečný J, Lama S.P., Cucchi M.E.C., Sosa M.A., Arenas G.N.
Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage.
Livestock Science 191, 191–195, 2016
  Rosero J.A., Killer J., Sechovcová H., Mrázek J., Benada O., Fliegerová K., Havlík J., Kopečný J.
Reclassification of Eubacterium rectale (Prévot et al., 1967) in a new genus Agathobacter gen. nov., as Agathobacter rectalis comb. nov., within the family Lachnospiraceae, and description of Agathobacter ruminis sp. nov., from the rumen
International Journal of Systematic and Evolutionary Microbiology, 66(2), 768-773, 2016
2014 Killer J., Švec P., Sedláček I., Černohlávková J., Benada O., Hroncová Z., Havlík J., Vlková E., Rada V., Kopečný J., Kofroňová O.
Vagococcus entomophilum sp. nov., from the digestive tract of wasp (Vespula vulgaris)
International Journal of Systematic and Evolutionary Microbiology 64 (3), 731-737, 2014
  Fliegerova K., Tapio I., Bonin A., Mrazek J., Callegari M.L., Bani P., Bayat A., Vilkki J., Kopečný J., Shingfield K.J., Boyer F., Coissac E., Taberlet P., Wallace R.J.
Effect of DNA extraction and sample preservation method on rumen bacterial population
Anaerobe, 29, 80 - 84, 2014
  Killer J., Havlík J., Vlková E., Rada V., Pechar R., Benada O., Kopečný J., Kofroňová O., Sechovcová H.
Lactobacillus rodentium sp. nov., from the digestive tract of wild rodents.
International Journal of Systematic and Evolutionary Microbiology, 64,(5), 1526 - 33, 2014
2013 Killer J., Mrázek J., Bunešová V., Havlik J., Koppová I., Benada O., Rada V., Kopečný J., Vlková E.,
Pseudoscardovia suis gen. nov., sp. nov., a new member of the family Bifidobacteriaceae isolated from the digestive tract of wild pigs (Sus scrofa).
Systematic & Applied Microbiol., 36, 11-16, 2013
  Klimešová K., Kverka M., Zákostelecká Z., Hrnčíř T., Hudcovic T., Štěpánková R., Rossman P., Rídl J., Kostovčík M., Mrázek J., Kopečný J., Tlaskalová H.,
Altered Gut Microbiota Promotes Colitis-Associated Cancer in IL-1 Receptor–Associated Kinase M–Deficient Mice.
Inflammatory Bowel Diseases,19, (6), 1266 – 1277, 2013
  Killer J., Sedláček I., Rada V., Havlík J., Kopečný J.,
Reclassification of Bifidobacterium stercoris (Kim et al., 2010) as a later heterotypic synonym to Bifidobacterium adolescentis.International Journal of Systematic and Evolutionary
Microbiology, 2013 63 (11): 4350-4353;
  Killer J., Ročková S., Bunešová V., Vlková E., Rada V., Kopecny J., Havlik J., Koppová I, Benada O., Mrázek J., Kofronová O., Profousová I.,
Alloscardovia macacae sp. nov., isolated from the milk of macaque (Macaca mulatta), emended and revised description of the genus Alloscardovia.
International Journal of Systematic and Evolutionary Microbiology, 63 (12), 4439 – 46, 2013
2012 Bunešová V., Domig K.J., Killer J., Vlková E., Kopečný J., Mrázek J., Ročková Š., Rada V.
Characterization of bifidobacteria suitable for probiotic use in calves,
Anaerobe 18, 166 – 168, 2012
  Vlčková K., Mrázek J., Kopečný j., Petrželková K.J.
Evaluation of different storage methods to characterize the fecal bacterial communities of captive western lowland gorillas (Gorilla gorilla gorilla).
J. of Microbiological Methods, 91, 45 – 51, 2012
2011 Zákostelská Z., Kverka M., Klimešová K., Rossmann P., Mrázek J., Kopečný J., Hornová M., Šrutková D., Hudcovič T., Ridl J., Tlaskalová-Hogenová H.,
Lysate of probiotic Lactobacillus casei DN-114 001 ameliorates colitis by strengthening the gut barrier function and changing the gut microenvironment
PLOS ONE 6 (11), e27961, 2011
  Kverka M., Zakostelska Z., Klimesova K., Sokol D., Hudcovic T., Hrncir T., Rossmann P., Mrazek J., Kopecny J., Verdu E., Tlaskalova-Hogenova H.,
Oral administration of Parabacteroides distasonis antigens attenuates experimental murine colitis through modulation of immunity and microbiota composition.
Clinical and Experimental Immunology 163 (2), 250 – 259, 2011