MVDr. Eva Nagyová, CSc., DSc.

Vzdělání

1976-1981 Vysoka škola veterinární, Košice, titul MVDr
1985 1. Atestace pro veterinární lékaře
1985-1990 vědecká aspirantura (CSc.) na téma: “Relationship between the level of cAMP and resumption of meiosis in mammalian oocytes”
Ústav živočišné fyziologie a genetiky AV ČR, Praha, Česká republika
2016 doktorská práce, (DSc.) na téma: “Cellular and molecular events associated with oocyte maturation in preovulatory follicles”;
Akademie věd České republiky, Praha, Česká republika

 

Zaměstnání a pracovní zařazení

1981-1985 Vedouci hygienik pro Interhotel Tatry , Stary Smokovec, Slovensko
1989-1992 Reprodukční endokrinologie, Výkumný ústav živočišné výroby, Praha, Česká republika
1993-dosud Vědecký pracovník - Ústav živočišné fyziologie a genetiky AV ČR, v. v. i., Liběchov
2004-dosud Vedoucí vědecký pracovník - Ústav živočišné fyziologie a genetiky AV ČR, v. v. i., Liběchov

 

Zahraniční pobyty a stáže

1992 Norwegian College of Veterinary Medicine, Oslo, Norsko
1994 INSERM, Clamart, Francie
1997 OECD Fellowship Award, Cancer Research Center, Ottawa, Kanada
2002 OECD Fellowship Award, Department of Public Health and Cell Biology, Faculty of Medicine, University of Rome, Itálie
2002-2007 Program vědecké a technologické spolupráce mezi vládou Itálie a vládou České republiky; - návštěvy vědců (6x3 měsíce)
2013 OECD Fellowship Award, Department of Public Health and Cell Biology, Faculty of Medicine, University of Rome, Itálie
2012–2014 Priority Research Collaboration mezi Slovenskou Akademií věd a Akademií věd České republiky (č. 18)

 

Hlavní vědecké zájmy

  • paracrinni faktory produkované oocyty a granulozními buňkami
  • ovulace: zrání oocytů, expanse kumulu a steroidogeneza
  • syntéza hyaluronanu, a její regulace gonadotropiny
  • interakce mezi TGFb/GDF9 and FSH/EGFR signálními dráhy
  • inhibice proteolýzy, receptoru pro progesteron a prostaglandinů
  • organizace kumulus-extracelulární matrix v předovulačním folikule
  • 2011-2013 Onkologický výzkum
    • Podporováno GlaxoSmithKline, s.r.o. Czech Republic
    • Nagyova et al., J Fertil Steril (2013)

 

Členství ve vědeckých společnostech

od roku 1997 Member of the "Society for Study of Reproduction"
od roku 2000 Member of "European Society of Human Reproduction and Embryology"
od roku 2012 Member of the "Society for Reproduction and Fertility"

 

Oponentury, expertizy

  • Pravidelné vypracovávání oponentských posudků pro: GAČR, GA AVČR, APVV, VEGA
  • 2003: Member of the Expert Group of EC /: Debating bioethical issues: Budapest
  • 2003-2004: Evaluator of EC/Individual Assessment Report in 2003;
    Panel evaluation process in Brussel in 2004
  • 2006,2008: Evaluator of Projects for “Cooperative Research Networks Ministry of Health and Consumer's Affairs”, Instituto de Salud Carlos III, Sinesio Delgado, 6, Madrid.
  • 2014: Evaluator for APVV project in Slovakia
  • 2015: "The Certificate of Peer Review Expert" from Slovak Research and Development Agency
  • Pravidelné recenze pro časopisy:
    • Biology of Reproduction
    • Reproduction
    • Molecular Reproduction Development
    • Animal Reproduction Science
    • Domestic Animal Endocrinology
    • Zygote
    • Human Reproduction
    • Fertility and Sterility
  • Oponent disertačních prací

 

Projekty (hlavní řešitel)

1994-1996 GA AV – (A745 401)
2001-2003 GA AV – (A5045102/01)
2005-2007 GA CR – (305/05/0960)
  Onkologický výzkum:
2011 Podporováno GlaxoSmithKline, s.r.o. Czech Republic
2013 Nagyova et al., J Fertil Steril

 

Projekty (spolupráce)

1998 GA CR-524/98/0231
2001 GA CR-504 5102/01
2003 Grant from the Italy Ministry of Education
2003-2007 Grant 52/ZV2 from Ministry of School, Youth and Education; Program of Scientific and Technological Co-operation /Italy-Czech Republic
2008-2011 GA CR 523/08/0111
2012-2014 Priority Research Collaboration between Slovak Academy of Sciences and Academy of Sciences of the Czech Republic
2011-2014 GA CR P502/11/0593

 

Vybrané publikace

Selection of publications (main author) in peer-reviewed journals

  • H index: 20; Total citations: 954: Average citation per article: 17 /WoS data: 24.8.2019/
  • 48 publications in peer-review journals, including 4 reviews as the sole author and 16 as the first author

 

  1. Cumulus Extracellular Matrix Is an Important Part of Oocyte Microenvironment in Ovarian Follicles: Its Remodeling and Proteolytic Degradation. Review. Eva Nagyová *, Lucie Nemcová and Antonella Camaioni. Int. J. Mol. Sci. 2022, 23(1), 54; https://doi.org/10.3390/ijms23010054 - 21 Dec 2021
  2. Nagyova E, Salustri A, Nemcova L, Scsukova S, Kalous J, Camaioni A. Versican G1 fragment establish a strongly stabilized interaction with hyaluonan-rich expanding matrix during oocyte maturation. Int J Mol Sci. 2020 Mar 25;21(7):2267. doi: 10.3390/ijms21072267.
  3. Nagyova E. The Biological Role of Hyaluronan-Rich Oocyte-Cumulus Extracellular Matrix in Female Reproduction. Int J Mol Sci 2018; 18; 19(1). doi: 10.3390/ijms19010283. Review Times Cited 4x
  4. Nagyova E, Nemcova L, Bujnakova Mlynarcikova A, Blaha M, Prochazka R, Scsukova S. Effect of bone morphogenetic protein-15 on gonadotropin-stimulated synthesis of hyaluronan and progesterone in porcine ovarian follicle. J Physiol Pharmacol 2017; 68(5):683-691 . Times Cited 4x
  5. Nagyova E, Kalous J, Nemcova L. Increased expression of pentraxin 3 after in vivo and in vitro stimulation with gonadotropins in porcine oocyte-cumulus complexes and granulosa cells. Domest Anim Endocrinol 2016l; 56:29-35. doi: 10.1016/j.domaniend.2016.01.004. Times Cited 1x
  6. Nagyova E. Organization of the expanded cumulus-extracellular matrix in preovulatory follicles: a role for inter-alpha-trypsin inhibitor. Endocr Regul 2015; 49(1):37-45. doi: 10.4149/endo_2015_01_37. Review Times Cited 8x
  7. Nagyova E, Scsukova S, Kalous J, Mlynarcikova A. Effects of RU486 and indomethacin on meiotic maturation, formation of extracellular matrix, and progesterone production by porcine oocyte-cumulus complexes. Domest Anim Endocrinol 2014; 48:7-14. doi: 10.1016/j.domaniend.2014.01.003. Times Cited 5x
  8. Nagyova E, Nemcova L, Mlynarcikova A, Scsukova S, Kalous J. Lapatinib inhibits meiotic maturation of porcine oocyte-cumulus complexes cultured in vitro in gonadotropin-supplemented medium. Fertil Steril 2013; 99(6):1739-48. doi: 10.1016/j.fertnstert.2012.12.040. Times Cited 3x
  9. Nagyova E. Regulation of cumulus expansion and hyaluronan synthesis in porcine oocyte-cumulus complexes during in vitro maturation. Endocr Regul 2012; 46(4):225-35.doi:10.4149/endo_2012_04_225. Review Times Cited 20x
  10. Nagyova E, Scsukova S, Nemcova L, Mlynarcikova A, Yi YJ, Sutovsky M, Sutovsky P. Inhibition of proteasomal proteolysis affects expression of extracellular matrix components and steroidogenesis in porcine oocyte-cumulus complexes. Domest Anim Endocrinol 2012;42(1):50-62. doi: 10.1016/j.domaniend.2011.09.003. Times Cited15x
  11. Nagyova E, Camaioni A, Scsukova S, Mlynarcikova A, Prochazka R, Nemcova L, Salustri A. Activation of cumulus cell SMAD2/3 and epidermal growth factor receptor pathways are involved in porcine oocyte-cumulus cell expansion and steroidogenesis. Mol Reprod Dev 2011; 78(6):391-402. doi: 10.1002/mrd.21312. Times Cited19x
  12. Nagyova E, Nemcova L, Prochazka R. Expression of tumor necrosis factor alpha-induced protein 6 messenger RNA in porcine preovulatory ovarian follicles. J Reprod Dev. 2009;55(3):231-5. doi: 10.1262/jrd.20115. Times Cited 17x
  13. Nagyova E, Camaioni A, Prochazka R, Day AJ, Salustri A. Synthesis of tumor necrosis factor alpha-induced protein 6 in porcine preovulatory follicles: a study with A38 antibody. Biol Reprod 2008;78(5):903-9. doi: 10.1095/biolreprod.107.064832. Times Cited 13x
  14. Nagyova E, Camaioni A, Prochazka R, Salustri A. Covalent transfer of heavy chains of inter-alpha-trypsin inhibitor family proteins to hyaluronan in in vivo and in vitro expanded porcine oocyte-cumulus complexes. Biol Reprod 2004;71(6):1838-43. doi: 10.1095/biolreprod.104.029595. Times Cited 37x
  15. Nagyova E, Vanderhyden BC, Prochazka R. Secretion of paracrine factors enabling expansion of cumulus cells is developmentally regulated in pig oocytes. Biol Reprod 2000;63(4):1149-56. doi: 10.1095/biolreprod63.4.1149. Times Cited 32x
  16. Nagyova E, Prochazka R, Vanderhyden BC. Oocytectomy does not influence synthesis of hyaluronic acid by pig cumulus cells: retention of hyaluronic acid after insulin-like growth factor-I treatment in serum-free medium. Biol Reprod 1999; 61(3):569-74. doi: 10.1095/biolreprod61.3.569. Times Cited 35x

Invited conferences and presentations:

  1. Proper structure of oocyte-cumulus extracellular matrix depends on the interaction between hyaluronan and inter-alpha-trypsin inhibitor in ovarian follicles. By: Nagyova E, Vodicka P. Fertility Conference, 9-11 January, 2020 EICC Edinburgh, UK
  2. Effect oocyte factors on granulosa cells differentiation and cumulus- extracellular matrix organization. By: Nagyova et al.  Fertility Conference, January 2019; ICC Birmingham, UK;
  3. Cumulus extracellular matrix represents an important new mechanism in the regulation of ovulatory process in porcine follicles. By: Nagyova Eva.  Fourth World Congress of Reproductive Biology 2017; Okinawa, Japan
  4. Effect of bone morphogenetic proetin15 (BMP15) on gonadotropin –stimulated synthesis of hyaluronan and progesterone in ovarian follicle By: Nagyova Eva, Fertility 2017 Meeting; Edinburg, UK
  5. Gonadotropins induce expression of versican in porcine oocyte–cumulus extracellular matrix and mural granulosa cells. By: Nagyova et al.  SRF Annual Conference, University of Winchester, Winchester, UK, 11-13 July, 2016
  6. Lapatinib inhibits meiotic maturation of porcine oocyte-cumulus complexes cultured in vitro in gonadotropin-supplemented medium By: Nagyova et al. Scientific Conference on Endocrine Disruptors; Bratislava, Slovakia, 16-17, 2012
  7. Involvement of EGFR and Smad2/3 Activation in FSH-Induced Porcine Oocytes-Cumulus Cell Complex Maturation By: Nagyova et al. Conference: 43rd Annual Meeting of the Society-for-the-Study-of-Reproduction; Milwaukee, WI; JUL 31-AUG 03, 2010; BIOL REPROD Suppl: 
  8. Altered organization of the oocyte-cumulus extracellular matrix in the presence of proteasomal inhibitor MG132 By: Nagyova et al. Conference: Joint Conference of the 33rd FEBS Congress/11th IUBMB Conference; Athens, GREECE; JUN 28-JUL 03, 2008. FEBS JOURNAL Suppl: 261
  9. Proteasomal activity is involved in oocyte meiosis and cumulus expansion during in vitro maturation of porcine oocytes.By: Yi, Young-Joo; Nagyova et al. Conference: 39th Annual Meeting of the Society-for-the-Study-of-Reproduction: Univ Nebraska Med Ctr, Omaha, NE; JUL 29-AUG 01, 2006. BIOL REPROD Special Issue:  177
  10. /Synthesis of tumor necrosis factor-stimulated gene-6 protein by cumulus and granulosa cells in porcine preovulatory follicle. By: Nagyova et al. Conference: 38th Annual Meeting of the Society-for-the-Study-of-Reproduction; Quebec City, CANADA; JUL 24-27, 2005. BIOL REPROD Special Issue: SI; 159
  11. Covalent transfer of inter-alpha-trypsin inhibitor heavy chains to hyaluronan in porcine oocyte-cumulus complexes cultured in vitro. By: Nagyova et al. Conference: 36th Annual Meeting of the Society-for-the-Study-of-Reproduction; CINCINNATI, OHIO; JUL 19-22, 2003 BIOL REPROD;   
  12. Evaluation of members of the TGF beta superfamily as candidates for the oocyte factors that control mouse cumulus expansion and steroidogenesis. By: Vanderhyden, BC; Macdonald, EA; Nagyova, E; Conference: 6th International Symposium on Reproduction; CRIEFF, SCOTLAND AUG 14-17, 2002. REPRODUCTION Suppl: 61; 55-70
  13. Effect of porcine oocytes at different stages of development on synthesis of hyaluronic acid by mouse oocytectomized complexes By: Nagyova, E et al. Conference: Proceedings of the Annual Conference of the International Embryo Transfer Society Location: Maastricht, Netherlands Date: January 09-11, 2000. Theriogenology 53 Issues:  462
  14. Porcine oocytes produce CEEF only during their growth period and transition to metaphase I. By: Nagyova et al. J. Annual Conference of the International Embryo Transfer Society Location: Nice, France Date: January 12-14, 1997.Theriogenology Volume: 47   Issue: 1   Pages: 197