Research experience and education
| 2017-now | postdoctoral research scientist (part-time), Laboratory of biochemistry and molecular biology of germ cells, Institute of Animal Physiology and Genetics CAS, v.v.i, Libechov, Czech Republic |
| 2015-now | postdoctoral research fellow, Laboratory of early mammalian development, Department of molecular biology and genetics, Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic |
| 2011-2015 | Ph.D. student, Epigenetic Programme, Babraham Institute, University of Cambridge, Cambridge, United Kingdom Ph.D. thesis: Defining the oocyte transcriptome and its relationship to de novo DNA methylation |
| 2010-2011 | MSc. student, Department of genetics, molecular biology and virology, Faculty of Science, Charles University, Prague, Czech Republic Master’s thesis: Evaluation of the use of cell-free microRNAs in maternal plasma in non-invasive prenatal diagnosis of placental insufficiency-associated complications and Down syndrome (at Department of molecular biology and cell pathology, 3rd Medical faculty, Charles University, Prague, Czech Republic) |
| 2009-2010 | visiting MSc. student, Department of Genetics, Cambridge University, Cambridge, United Kingdom Master’s project: Evaluation of methods of direct mutation and haplotype analysis for non-invasive prenatal diagnosis of tuberous sclerosis from cell-free fetal DNA in maternal plasma (at Cambridge Institute for Medical Research, Cambridge University, Cambridge, United Kingdom) |
| 2006-2009 | Bachelor’s degree in Biology |
Additional scientific training
| 9/2015 | Animal training course, validated by licence to manage and perform experiments with animals (Ministry of Agriculture, Czech Republic) |
| 7/2015 | Confocal Microscopy and Image Analysis Workshop (Department of Molecular Biology, University of South Bohemia, Czech Republic) |
| 10/2013 | Whole Exome Sequencing and RNA-seq Data Analysis course (Instituto Gulbenkian de Ciencia, Oeiras, Portugal) |
| 7/2013 | An Introduction to Solving Biological Problems with R (Graduate School of Life Sciences, University of Cambridge, United Kingdom) |
| 12/2012 | Next Generation Sequencing course (Graduate School of Life Sciences, University of Cambridge, United Kingdom) |
| 10/2011 | Home Office Animal Training Course (course validated by licence to manage and perform experiments with animals, Cambridge Home Office, United Kingdom) |
Selected talks and poster presentations
A total of 14 talks and 8 poster presentations
| 9/2018 | Visegrad Group Society for Developmental Biology Inaugural Meeting (Brno, Czech Republic) Talk (selected abstract): Stage specific mTOR-regulated translation affects the relative spatial positioning of cells during mouse preimplantation embryo development. |
| 4/2017 | EMBO Workshop Awakening the genome: The maternal-to-zygotic transition (Dresden, Germany) Talk (selected abstract): mTOR-regulated translation affects spatial cellular positioning in mouse preimplantation embryos |
| 9/2016 | RNA Club 2016 (Brno, Czech Republic) Talk (selected abstract): Determinants of dynamics of DNA methylation establishment in oocytes |
| 12/2014 | Max Planck Freiburg Epigenetics Meeting (Freiburg, Germany) Poster: Promoter activity determines DNA methylation landscape in oocytes. |
| 9/2014 | RNA Club 2014 (Prague, Czech Republic) Talk (selected abstract): Activity of promoters shapes DNA methylation landscape in oocytes |
| 5/2014 | European Human Genetics Conference 2014 (Milan, Italy) Poster: Transcription as a key determinant of DNA methylation landscape in oocytes |
| 4/2014 | Keystone Symposium on Epigenetic Programming and Inheritance (Boston, Massachusetts, USA) Poster: Transcription as a key determinant of DNA methylation landscape in oocytes |
| 11/2013 | Mammalian Genome and Development Workshop (London, United Kingdom) Talk (selected abstract): Global identification of active transcriptional start sites in germ cells and correlation with CpG methylation |
Publications
| 2018 | Koncicka M., Tetkova A., Jansova D., Del Llano E., Gahurova L., Kracmarova J., Prokesova S., Masek T., Pospisek M., Bruce A.W., Kubelka M., Susor A.: Increased expression of maturation promoting factor components speeds up meiosis in oocytes from aged females. Int J Mol Sci. 2018, 19(9):2841. |
| Hanna C.W., Taudt A., Huang J., Gahurova L., Kranz A., Andrews S., Dean W., Stewart A.F., Colome-Tatche M., Kelsey G.: MLL2 conveys transcription-independent H3K4 trimethylation in oocytes. Nat Struct Mol Biol. 2018, 25(1):73-82. | |
| 2017 | Brici D., Zhang Q., Reinhardt S., Dahl A., Hartmann H., Schmidt K., Goveas N., Huang J., Gahurova L., Kelsey G., Anastassiadis K., Stewart A.F., Kranz A.: Setd1b, encoding a histone 3 lysine 4 methyltransferase, is a maternal effect gene required for the oogenic gene expression program. Development. 2017, 144(14):2606-2617. |
| Gahurova L.*, Tomizawa S.I.*, Smallwood S.A., Stewart-Morgan K.R., Saadeh H., Kim J., Andrews S.R., Chen T., Kelsey G.: Transcription and chromatin determinants of de novo DNA methylation timing in oocytes. Epigenetics Chromatin. 2017, 10:25.* equal contribution | |
| 2016 | Stewart K.R.*, Veselovska L.*, Kelsey G.: Establishment and functions of DNA methylation in the germline. Epigenomics. 8(10):1399-1413. * equal contribution |
| 2015 | Stewart K.R., Veselovska L., Kim J., Huang J., Saadeh H., Tomizawa S-I., Smallwood S.A., Chen T., Kelsey G.: Dynamic changes in histone modifications precede de novo DNA methylation in oocytes. Genes Dev. 29(23):2449-2462. |
| Veselovska L.*, Smallwood S.A.*, Saadeh H., Stewart K.R., Krueger F., Maupetit-Méhouas S., Arnaud P., Tomizawa S-I., Andrews S., Kelsey G.: Deep sequencing and de novo assembly of the mouse oocyte transcriptome define the contribution of transcription to the DNA methylation landscape. Genome Biol. 16:209. * equal contribution |