Ing. Chahrazed Mekadim, Ph.D.

Date of birth: 12/07/1986
Nationality: Algerian

Education

2004-2009 State engineer in Biotechnology
University of Oran, “Ahmed ben Bella” Oran (Algeria)
Option: Microbiological Engineering
2009-2013 Post graduation (Magister) in Biotechnology
University of science and technology of Oran (USTO) ”Mohamed Boudiaf”, Oran (Algeria)
Option: Cytochemistry, Structure, modelling and simulation of macromolecules in medical and agronomic interest
2015-now Ph.D. Study
Czech University of Life Sciences Prague (CULS), Prague (Czech Republic)
Faculty of Agrobiology, Food and Natural resources
Department of Microbiology, Nutrition and Dietetics
Study program: Animal Sciences
Field: General: Animal Sciences
Training Facility: Laboratory of Anaerobic Microbiology
Institute of Animal Physiology and Genetics Czech Academy of Sciences

Professional experience

2012-2014

Biologist in a medical laboratory analysis
BENSMAINE'S laboratory

  • Serology
  • Hemobiology
  • Bacteriology
2017-now Laboratory of Anaerobic Microbiology
Institute of Animal Physiology and Genetics Czech Academy of Sciences

University teaching

  • University of sciences and technology of Oran (USTO) "Mohamed BOUDIAF"
    Subject: molecular modelling, simulation and technology
  • University of Oran, “Ahmed Ben Bella” Oran (Algeria)
    Subject: Microbiology
  • Czech University of Life Sciences Prague (CULS), Prague (Czech Republic)
    Subject: Microbiology

Major scientific interests

  • Classical microbiological methods of bacterial identification and anaerobic culture.
  • MALDI-TOF
  • DNA extraction and purification
  • PCR, qPCR, DGGE
  • NGS (Next Generation Sequencing) Ion Torrent.

Digital competence

  • Mastery of Software of sequences and phylogenetic analysis (BioEdit, MEGA, Genious,…)
  • BioNumerics (fingerprint analysis)
  • Initiation in microbiome analysis: QIIME, C4Sys HTS course for Amplicon data analysis (SEED2)

Publications

2018 Mekadim C, Killer J, Mrázek J, Bunešová V, Pechar R, Hroncová Z, Vlková E. Evaluation of the infB and rpsB gene fragments as genetic markers intended for identification and phylogenetic analysis of particular representatives of the order Lactobacillales. Archives of Microbiolgy, 2018.200(10):1427-1437.
doi: 10.1007/s00203-018-1554-7.
  Mekadim C, Killer J, Pechar R, Mrázek J. Fragment of the aspartul-tRNA synthetase applicable as a shared classification and phylogenetic marker in particular representatives of the order Lactobacillales. Folia Microbiologica (Praha), 2018.
doi: 10.1007/s12223-018-0638-8.
  Mekadim C, Killer J, Pechar R, Mrázek J. Variable regions of the glyS, infB and rplB genes usable as novel genetic markers for identification and phylogenetic purposes of genera belonging to the family Propionibacteriaceae. International journal of Systematic and Evolutionary Microbiology, 2018. 68 (8): 2697-2705.  
doi: 10.1099/ijsem.0.002873.
  Killer J, Mekadim C, Pechar R, Bunesova V, Vlková E. The threonine-tRNA ligase gene region is applicable in classification, typing, and phylogenetic analisis of bifidobacteria. Journal of Microbiology, 2018. (10):713-721.
doi: 10.1007/s12275-018-8167-3
  Killer J, Mekadim C, Pechar R, Bunesova V, Mrázek J, Vlková E. Gene encoding the CTP synthetase as an appropriate molecular tool for identification and phylogenetic study of the family Bifidobacteriaceae. MycrobiologyOpen, 2018. 7(4):e00579.
doi: 10.1002/mbo3.579.
2017 Pechar R, Killer J, Mekadim C, Geigerová M, Rada V. Classification of Culturable Bifidobacterial Population from Colonic Samples of Wild Pigs (Sus scrofa) Based on Three Molecular Genetic Methods. Current Microbiology, 2017.74(11):1324-1331.
doi: 10.1007/s00284-017-1320-0.
  Sechovcová H, Killer J, Pechar R, Geigerová M, Švejstil R, Salmonová H, Mekadim C, Rada V, Vlková E, Kofroňová O, Benada O. Alloscardovia venturai sp. nov., a fructose 6 - phosphate phosphoketolase-positive species isolated from the oral cavity of a guinea-pig (Cavia aperea f. porcellus).  International Journal of Systematic and Evolutionary Microbiology, 2017. 67(8):2842-2847.
doi: 10.1099/ijsem.0.002031.
  Killer J, Pechar R, Švec P, Salmonová H, Švejstil R, Geigerová M, Rada V, Vlková E, Mekadim C. Lactobacillus caviae sp. nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea-pig (Cavia aperea f. porcellus).  International Journal of Systematic and evolutionary Microbiology, 2017. 67(8):2903-2909.
doi: 10.1099/ijsem.0.002044.