Mgr. Denisa Jansová, Ph.D.

Date of birth:  20th July 1988

Education/Qualifications

2013-2017 Charles University in Prague, Czech Republic; Faculty of Science
A Ph.D. student at the Developmental Biology Program
Research interest: Transcriptome in the mammalian germ cells
Ph.D. Thesis: Identification of key regulators of gene expression in mammalian oocyte and embryo
2011-2013 Charles University in Prague, Czech Republic; Faculty of Science Department of Cell Biology,
Master's study program
Diploma Thesis: Transcriptional activity of the genes characterizing developmentally competent cytoplasm in bovine oocytes
2008-2011 Charles University in Prague, Czech Republic; Faculty of Science Department of Cell Biology,
Bachelor's study program
Bachelor's thesis: Actin and its regulation in clathrin endocytosis

 

Employment to Date/Work Experience

6/2013-10/2013 Institute of Animal Physiology and genetic AS CR v.v.i.
Post: research assistant V1

 

Skills

  • Microscopy (epifluorescence, confocal)
  • In vitro cultivation of mammalian oocyte and embryos
  • Fluorescence immunocytochemistry and western blot analysis
  • Fluorescence in situ hybridization of RNA
  • Proximity ligation assays
  • Gene expression (real-time PCR)
  • Data analysis and interpretation

 

Other Experience/Presentation and conferences

09/2018 RNA CLUB 2018, Prague, IMG, oral presentation: RNA biosensors in living cells - Spatiotemporal mRNA translation in mitosis
09/2017 EMBO conference „Protein Synthesis and Translational Control“, Heidelberg, Germany, participant poster session: RNA a translation machinery in oocyte and embryo
07/2017 RNA localization and local translation 2017, Barna, Italy, participant poster session: RNA a translation machinery in oocyte and embryo
09/2016 RNA CLUB 2016, CEITEC Brno, Czech Republic, oral presentation, RNA world in progress: Complex visualization of transcriptome in oocyte and embryo
09/2015 EMBO conference „Protein Synthesis and Translational Control“, Heidelberg, Germany, participant poster session: Phosphorylation of 4E-BP1 regulates translation in mammalian germ cells
09/2014 18th International Microscopy congress (Prague), participant poster session Phosphorylation of 4E-BP1 promotes translation at the oocyte spindle
05/2014 Summer school of microinjection and advance microscopy technique (CEITEC, Brno)
2010-2011 Charles University in Prague, Czech Republic; Faculty of Science
Department of Cell Biology
Searching of candidate genes by RNAi in Wnt signaling on C. elegans

 

Fundings

4/2018 EMBO Short-Term Fellowship, Switzerland, FMI, training of SunTag   method, Jeffrey Chao lab
07/2017 EMBO Boehringer Ingelheim Fonds Travel Grant
09/2015 EMBO travel grant: Protein Synthesis and Translational Control
04/2015-12/2017 GAUK 227026, student’s grant of Charles Universtity: RNA world in mammalian oocyte
04/2014-12/2014 IGA, internal student’s grant of the Institute of Animal Physiology and Genetics: Localization specific mRNA in live oocyte

 

Publications

2018 Koncicka M, Tetkova A, Jansova D, Del Llano E, Gahurova L, Kracmarova J, Prokesova S, Masek T, Pospisek M, Bruce A, Kubelka M , Susor A . Maturation promoting factor activation in oocytes from aged females speeds up meiosis, IJMS, 2018. IF:3,68
  Jansova D, Koncicka M, Tetkova A, Susor A. Transcriptome and translation in mammalian oocyte and embryo, Plos One. 2018. IF:2,85
2017 Jansova D, Koncicka M, Tetkova A, Cerna R, Malik R, Llano del E, Kubelka M, Susor A. Regulation of 4E-BP1 activity in the mammalian oocyte, Cell Cycle, 2017. IF: 3,95
2016 Nemcova L, Jansova D, Vodickova-Kepkova K, Vodicka P, Jeseta M, Machatkova M, Kanka J. Detection of genes associated with developmental competence of bovine oocytes. Anim Reprod Sci. 2016. IF: 1,377
  Susor A, Jansova D, Anger M, Kubelka M. Translation in the mammalian oocyte in space and time. Cell Tissue Res. 2016. Review. IF:2,948
2015 Jansova D, Single Molecule RNA FISH in the mammalian oocyte. Bio-protocol. 2015.
  Susor A, Jansova D, Cerna R, Danylevska A, Anger M, Toralova T, Malik R, Supolikova J, Cook MS, Oh JS, Kubelka M. Temporal and spatial regulation of translation in the mammalian oocyte via the mTOR-eIF4F pathway. Nat Commun. 2015. IF:11,329