DEPARTMENT OF REPRODUCTIVE AND DEVELOPMENTAL BIOLOGY
Laboratory of Biochemistry and Molecular Biology of Germ Cells

Institute of Animal Physiology and Genetics, v.v.i.

Academy of Sciences of the Czech Republic

277 21 Libechov

Czech Republic


Phone: +420 315 639 582
Fax: +420 315 639 510


E-mail: kovarova@iapg.cas.cz

1967 – 1972	Faculty of Natural Sciences, Charles University, Prague, M.Sc. in Chemistry
1972 - 1976	Faculty of Natural Sciences, Charles University, Prague, Faculty of Medicine, Charles University, Hradec Kralove, Ph.D. in Biochemistry
1976 - 1989	Research Assistant and Lecturer, Department of Biochemistry, Faculty of Medicine, Charles University, Hradec Kralove
1989 - 1993	Senior Scientist and Lecturer, Department of Biochemistry, Faculty of Medicine, Charles, University, Hradec Kralove
1993 – 2002	Senior Scientist, Leader Group, Institute of Radiobiology and Immunology , Purkyne Military Medical Academy, Hradec Kralove
2002 -	Senior Scientist, Department of Reproduction, Institute of Animal Physiology and Genetics, v.v.i., Academy of Sciences of the Czech Republic, Libechov

• Proteomes of germ cells/oocytes during in vitro maturation
• Proteomic analysis of neural stem cells: stemness and differentiation
• Identification of candidate biomarkers of anti-cancer effect of synthetic cyclin-dependent inases inhibitor
• Posttranslational modifications of proteins as modulators of protein properties and activities

• Purine inhibitors of cyclin-dependent kinases as anticancer and antimitotic compounds
  (Grant Agency of the Czech Republic, 2002-2004 )
• Proteomic and phosphoproteomic analysis of meiotic division of oocytes
  (Grant Agency of the Czech Republic, project leader, 2004-2006)
• New generations of cyclin-dependent kinase inhibitors with significant anticancer properties
  (Grant Agency of the Czech Republic, 2005- 2007)
• Female gametes – regulation of their meiotic and developmental competence and posibilities of its practical application
  (Grant agency of Czech Academy of Sciences, project leader, 2005-2009)

• Cofounder and member of European Proteomic Asociation ( EuPA)
• member of Czech Society for Biochemistry and Molecular biology
• president of Czech Proteomic Society
• member of Swiss Proteomic Society
• Member of Editorial board of the “Proteomics”
• Member of Editorial board of the „Technology in Cancer Research and Treatment“
• Member of Editorial board of the „Current Analytical Chemistry“
• Chair of the Scientific Board of the Institute of Animal Physiology and Genetics, v.v.i., Academy of Sciences of the Czech Republic

1994	Czech Society for Clinical Chemistry Award
2001	The Wellcome Trust Travel Award
2005	Award for the best publication of IAPG AV CR in 2004
2005	Nomination for 6th Royan International Award

• Vodička, Petr - Skalníková, Helena - Kovářová, HanaThe characterization of stem cell proteomes. Current Opinion in Molecular Therapeutics. Roč. 8, č. 3 (2006), s. 232-239. ISSN 1464-8431
• Šušor, Andrej - Ellederová, Zdeňka - Jelínková, Lucie - Halada, Petr - Kavan, Daniel - Kubelka, Michal - Kovářová, Hana Proteomic Analysis of Porcine Oocytes During in vitro Maturation Reveals Essential Role for the Ubiquitin C- terminal hydrolase-L1. Reproduction. Roč. 134, č. 4 (2007), s. 559-568. ISSN 1470-1626
• Skalníková, Helena - Řehulka, Pavel - Chmelík, Josef - Martinková, Jiřina - Zilvarová, Michaela - Gadher, S. J. - Kovářová, Hana Relative quantitation of proteins fractionated by the ProteomeLab™ PF 2D system using isobaric tags for relative and absolute quantitation (iTRAQ). Analytical and Bioanalytical Chemistry. Roč. 389, č. 5 (2007), s. 1639-1645. ISSN 1618-2642
• Skalníková, Helena - Halada, Petr - Vodička, Petr - Motlík, Jan - Řehulka, Pavel - Horning, O. - Chmelík, Josef - Norregaard Jensen, O. - Kovářová, Hana A proteomic approach to studying the differentiation of neural stem cells. Proteomics. Roč. 7, 11 (2007), s. 1825-1838. ISSN 1615-9853
• Ellederová, Zdeňka - Cais, Ondřej - Šušor, Andrej - Uhlířová, Kateřina - Kovářová, Hana - Jelínková, Lucie - Tomek, W. - Kubelka, Michal ERK 1/2 kinase metabolic pathway is responsible for phosphorylation of translation initiation factor eIF4E during in vitro maturation of pig oocytes. Molecular Reproduction and Development. Roč. 75, č. 2 (2007), s. 309-317. ISSN 1040-452X
• Hajdúch, M. - Skalníková, Helena - Halada, Petr - Vydra, D. - Džubák, P. - Dziechciarková, M. - Strnad, M. - Radioch, D. - Gadher, S. J. - Kovářová, Hana Cyclin-dependent kinase inhibitors and cancer: usefulness of proteomic approaches in assessment of the molecular mechanisms and efficacy of novel therapeutics. Clinical Proteomics. From Diagnosis to Therapy. Weinheim : Wiley, 2007 - (Van Eyk, J.; Dunn, M.) S. 177-202. ISBN 978-3-527-31637-3
• Ellederova Z, Kovarova H, Melo-Sterza F, Livingstone M, Tomek W, Kubelka M. Suppression of translation during in vitro maturation of pig oocytes despite enhanced formation of cap-binding protein complex eIF4F and 4E-BP1 hyperphosphorylation. Mol Reprod Dev. 2006 Jan;73(1):68-76.
• Baran V, Kovarova H, Klima J, Hozak P, Motlik J. Re-localization of nuclear DNA helicase II during the growth period of bovine oocytes. Histochem Cell Biol. 2006 Jan;125(1-2):155-64. Epub 2005 Sep 27.
• Skalnikova H, Halada P, Dzubak P, Hajduch M, Kovarova H. Protein fingerprints of anti-cancer effects of cyclin-dependent kinase inhibition: identification of candidate biomarkers using 2-D liquid phase separation coupled to mass spectrometry. Technol Cancer Res Treat. 2005 Aug;4(4):447-54.
• Ellederova Z, Halada P, Man P, Kubelka M, Motlik J, Kovarova H. Protein patterns of pig oocytes during in vitro maturation. Biol Reprod. 2004 Nov;71(5):1533-9. Epub 2004 Jun 30.
• Golovliov I, Baranov V, Krocova Z, Kovarova H, Sjostedt A. An attenuated strain of the facultative intracellular bacterium Francisella tularensis can escape the phagosome of monocytic cells. Infect Immun. 2003 Oct;71(10):5940-50.
• Kovarova H, Hajduch M, Livingstone M, Dzubak P, Lefkovits I. Analysis of state-specific phosphorylation of proteins by two-dimensional gel electrophoresis approach. J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Apr 5;787(1):53-61.

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