DEPARTMENT OF VERTEBRATE EVOLUTIONARY BIOLOGY AND GENETICS
Laboratory of Fish Genetics

Institute of Animal Physiology and Genetics, v.v.i.

Academy of the Sciences of the Czech Republic

Rumburska 89

277 21 Libechov

Czech Republic



Phone: +420 315 639 545
Fax: +420 315 639 510
E-mail: kotlik@iapg.cas.cz

1991 - 1993	Bachelor; Faculty of Natural Sciences, Charles University, Prague
1993 - 1996	Magister, Faculty of Natural Sciences, Charles University, Prague
1998 – 1999	researcher, department of Zoology, Faculty of Natural Sciences, Charles University, Prague
1999 – present	researcher, Laboratory of Fish Genetics, IAPG AS CR, Libechov
2000 - 2001	Docteur du Museum National d’Histoire Naturelle; 2001; Museum National d’Histoire Naturelle (MNHN), Paris, France „Phylogeographie et systematique des barbeaux d'Europe Centrale - Ph. D. thesis, Museum National d'Histoire Naturelle, Paris.

I am interested in estimating population and species evolutionary history on the basis of molecular genetic data, especially those that can be historically ordered and analysed from a genealogical perspective, such as within the phylogeographical framework. I am very enthusiastic about using nuclear genealogical data for phylogeographical analysis both to address new hypotheses and to assess predictions formulated on the basis of mitochondrial DNA phylogeographical patterns. My research experience includes generating historically ordered mitochondrial as well as unordered nuclear genetic data using various technologies (direct, automated DNA sequencing; PCR-RFLP analysis; PCR microsatellite genotyping; electrophoretic analysis of allozyme variation), and their analysis with different statistical methods of phylogeny (DNA evolutionary model selection, maximum likelihood, maximum parsimony, neighbor joining, Shimodaira-Hasegawa test of topologies, statistical parsimony) and population structure (F-statistics, nested clade analysis) and history inference (nested clade analysis, mismatch distribution). I am familiar with statistical packages/programs for genetic data analysis such as ARLEQUIN, GENETIX, GEODIS, MEGA, MODELTEST, PAUP, PHYLIP and TCS. I have got experience with field collecting and working with various Palaearctic vertebrate model organisms of freshwater fish (genera Barbus, Cobitis) and amphibians (Rana, Triturus), although I have no important preferences for my future work in this respect.

• Podil severnich a jiznich refugii na postglacialni kolonizaci, hlavni resitel, 2007-2009 GAAV IAA600450701

• Kotlik P, Markova S, Choleva L, Bogutskaya NG, Ekmekci FG, Ivanova PP (2008) Divergence with gene flow between Ponto-Caspian refugia in an anadromous cyprinid Rutilus frisii revealed by multiple gene phylogeography. Molecular Ecology, 17: 1076-1088.
• Sediva A, Janko K, Slechtova V, Kotlik P, Simonovic P, Delic A & Vassilev M (2008) Around or across the Carpathians: colonization model of the Danube basin inferred from genetic diversification of stone loach (Barbatula barbatula) populations. Molecular Ecology, 17: 1277-1292.
• Stuart P, Mirimin L, Cross TF, Sleeman DP, Buckley NJ, Telfer S, Birtles RJ, Kotlik P & Searle JB (2007) The origin of Irish bank voles Clethrionomys glareolus assessed by mitochondrial DNA analysis. The Irish Naturalists' Journal, 28, 440-446.
• Markova S, Dufresne F, Rees DJ, Cerny M, Kotlik P (2007) Cryptic intercontinental colonization in water fleas Daphnia pulicaria inferred from phylogenetic analysis of mitochondrial DNA variation. Molecular Phylogenetics and Evolution , 44, 42-52.
• Janko K, Bohlen J, Lamatsch DK, Flajshans M, Epplen JT, Rab P, Kotlik P, Slechtova V (2007) The gynogenetic reproduction of diploid and triploid hybrid loaches (Cobitis: Teleostei), and their ability to establish clonal lineages-on the evolution of polyploidy in asexual vertebrates. Genetica, 131, 185-194.
• Kotlik P, Berrebi P (2007) Nested clade phylogeographical analysis of barbel (Barbus barbus) mitochondrial DNA variation. In: Phylogeography of Southern European Refugia (eds. Weiss S, Ferrand N), pp. 315-325. Springer, Dordrecht, The Netherlands.
• Kotlik P, Deffontaine V, Mascheretti S et al. (2006) A northern glacial refugium for bank voles (Clethrionomys glareolus). Proceedings of the National Academy of Sciences of the United States of America, 103, 14860-14864.
• Janko K, Kotlik P, Culling M. A., Rab P. (2005): Ice age cloning - comparison of the Quaternary evolutionary histories of sexual and clonal forms of spiny loaches (Cobitis; Teleostei) using the analysis of mitochondrial DNA variation. Molecular Ecology. 14: 2991-3004.
• Deffontaine V, Libois R, Kotlik P, et al. (2005): Beyond the Mediterranean peninsulas: evidence of central European glacial refugia for a temperate forest mammal species, the bank vole (Clethrionomys glareolus) Molecular Ecology 14 (6): 1727-1739.
• Kotlik, P, Bogutskaya N.G., Ekmekci F.G., 2004: Circum Black Sea phylogeography of Barbus freshwater fishes: divergence in the Pontic glacial refugium. Molecular Ecology 13, 87-95.
• Janko K., Kotlik, P, Rab P., 2003: Evolutionary history of asexual hybrid loaches (Cobitis: Teleostei) inferred from phylogenetic analysis of mitochondrial DNA variation. Journal of Evolutionary Biology 16, 1280-1287.

All publications via PubMed