Employees

Projects

Šimůnek Jiří, MVDr., CSc.


Function:
Profession:vědecký pracovník
E-mail:
Telephone: +420 267 090 509
Mobile phone:
Department: Laboratory of Anaerobic Microbiology
Building: Praha-Krč/
Institutional service phone:
Web:

Curriculum vitae:

1976-1982

Veterinary and Pharmaceutical University Brno

1983-1989

Specialist, Institute of Animal Physiology and Genetics, AS CR, v.v.i., Prague

1989

PhD Thesis: Manipulation of the Rumen  Fermentation

1989-2000

Research worker, Institute of Animal Physiology and Genetics, AS CR, v.v.i., Prague

2000-present

Independent research worker, Institute of Animal Physiology and Genetics, AS CR, v.v.i., Prague

Specialization:

Microbiology and biochemistry of anaerobic bacteria from the animal and human digestive tract, chitin enzyme degradation, chitinases, chitinolytic bacteria of the human and animal gastrointestinal tract.

Awards:

2009

3rd Award Prize for poster presentation, conference EUCHIS 2009, Venice

2011

3rd Award Prize for poster presentation, conference EUCHIS 2011, St. Petersburg

Internship abroad:

1987

Institute of Animal Physiology and biochemistry, Lviv, Ukraine

1988

Institute of Animal Physiology, Alma-Ata, Kazakhstan

1989

Rowett Research Institute, Aberdeen, Scotland, U.K.

1989

The Kielanowski Institute of Animal Physiology and Nutrition, Jablona, Poland

Publications:

2007

Kopečný J., Šimůnek J.: Chitosan application in food preparation and digestion. Adv. Chitin Sci. X, Senel S., Varum KM, Sumnu MM, Hincal AA, Eds., alp Offset, Ankara, pp. 467-473

2008

Belzecki G., Miltko R., Michalowski T., Šimůnek J., Kopečný J.: chitinolytic aktivity of the sheep rumen ciliate Diploplastron affine. Folia Microbiol. 53: 201-203

2008

Šimůnek J., Tishchenko G., Koppová I.: Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan, Folia Microbiologica 53:249-254

2010

Lukáš F., Šimůnek J., Mrázek J., Kopečný J.: PCR-DGGE Analysis of bacterial population attached to the bovine rumen wall. Folia Microbiol. 55: 345-348

2010

Tishchenko G., Koppová I.: Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan, Folia Microbiologica 53:249-254

2010

Šimůnek J., Koppová I., Lukáš F., Tishchenko G., Belzecki G.: The antimicrobial action of low-molar-mass chitosan, chitosan derivatives and chitooligosaccharides on bifidobacteria. Folia Microbiol. 55: 379-382

2010

Tishchenko G., Koppová I., Šimůnek J., Dohnálek J.: Extracellular comlex of chitinolytic enzymes of Clostridium paraputrificum strain J4 separated by membrane ultrafiltration, Folia Microbiologica 55:386-389

2010

Mrázek J., Koppová I., Kopečný J., Šimůnek J., Fliegerová K: PCR-DGGE based study of fecal microbial stability during the long-term chitosan supplementation of human. Folia Microbiologica 55:352-358

2011

Dušková J., Tishchenko G., Ponomareva E., Šimůnek J., Koppová I., Skálová T., Šťěpánková A., Hašek J., Dohnálek J.: Chitinolytic enzymes from bacterium inhabiting human gastrointestinal tract – critical parameters of protein isolation from anaerobic culture. Acta Biochima Polonica 58:261-263

2011

Tishchenko G., Šimůnek J., Brus J., Netopilík M., Pekárek M., Walterová Z., Koppová I., Lenfeld J.: Low-molecular-weight-chitosans: Preparation and characterization. Carbohydrate Polymers, 86:1077-1081

2011

Tishchenko G., Šimůnek J., Bartoňová H., Dušková J., Dohnálek J.: Sample preparation in separation of the extracellular chitinolytic enzymes of the human intestinal bacterium Clostridium paraputrificum  J4 from the culture fluids. J. Chromatography B, 879: 2175-2178

 

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