Employees

Projects

Kopečný Jan, Ing.,DrSc.


Function:
Profession:Director (Research Scientist)
E-mail:
Telephone: +420 267 090 502
Mobile phone:
Department: Laboratory of Anaerobic Microbiology
Building: Praha-Krč/39
Institutional service phone: 12
Web:

Curriculum vitae:

Education

1970 - 1975

Dipl. Ing., Biochemistry, Institute of Chemical Technology, Prague, Stability of lysosomes in meat post?mortem, supervisor: prof. ing. Jan Káš, DrSc

 

1977 - 1981

CSc., Biochemistry,  (eq. PhD), Czechoslovak Academy of Sciences,  In vitro incubation of rumen microbes,    supervisor: ing. Stanislav Bartoš, DrSc.

 

1985 - 1997

DSc., Microbiology, Institute of Animal Physiology and Genetics, Microbial Feed degradation in the Rumen.

Position

1991

Senior Scientific Officer

 

2003

Head of Laboratory of Anaerobic Microbiology

Specialization:

My studies started with rumen proteolytic, amylolytic and cellulolytic microbes and their hydrolytic enzyme production. Following years I was dealing with rumen microbes adhesion to feed particles. We introduced methods for anaerobic microbiology and developed probiotics for in vivo testing. Then we moved to biochemistry of rumen bacterium Butyrivibrio fibrisolvens and rumen fungi and interactions of rumen microbes in vitro and in vivo. It led to the development of enzymatic method for estimation of protein degradation in the rumen and feed digestibility for ruminants. I took a part in transformation of anaerobes. I was able with a shuttle vector with fluoroacetate dehalogenase to transform a many Pseudobutyrivibrio isolates. Transformation of undescribed isolates directed me to taxonomy of butyrate-producing bacteria and description of two new species: Butyrivibrio hungatei, Pseudobutyrivibrio xylanivorans. I was involved in description of Butyrivibrio proteoclasticus, later on.

Knowledge of butyrate-producing bacteria helped me to map changed microbiome in patients with the human bowel diseases: we described the effect of Inflammation Bowel Disease and Celiac Disease and we able to show the importance of probiotic treatment. It was possible to describe Bifidobacteria gut colonization in newborns, calves as well as in social insect digestive tract.

In last decade, we have detected presence of bifidobacteria in most vertebrates and many insect species. In frame of this, we described new bifidobacteria in the gut of bumblebees: Bifidobacterium bombi, B. bohemicum and B. actinicoloniformis and a new genus Bombiscardovia (Bombiscardovia coagulans).

Societies:

1999

Biotechnology Society of CR                      

1985

Czechoslovak Society for Microbiology

1983

Czech Society for biochemistry and Molecular biology  

1981-2003      

American Society for Microbiology

2009

Czech Society for Immunology

2009

Bioprospect, Editorial Board,                                          

2010

Folia Microbiologica, Editorial Board,

2012

Journal of Nutritional Ecology and Food Research, Editorial Board,

Grants:

Tlaskalová Helena, MBÚ, Kopečný J.,

Bacteria in etiology, prevention and therapy of experimentally induced intestinal inflammation and colorectal carcinoma.
GA ČR 304/11/1252, 2011 - 2014.

Wallace R. John, Rowett (UK), Kopečný J.,
Ruminomics: Connecting the animal genome, gastrointestinal microbiomes and nutrition to improve digestion efficiency and the environmental impacts of ruminant livestock production
FP7  289319,  2012 - 2015.

Němečková Irena, MILCOM, Kopečný J.,
Fermented dairy products and cheeses for healthy human nutrition, technological procedures of their production and evaluation methods accenting high level of microbiological safety and improved nutritional parameters.
NAZV QJ1510338, 2015 - 2019.

Kopečný J., 
Structure and synergism of fibrolytic enzymes in the rumen. 
Czech Science Foundation, 16-12431S, 2016 - 2018

Publications:

Kverka M., Zakostelska Z., Klimesova K., Sokol D., Hudcovic T., Hrncir T., Rossmann P.,

            Mrazek J., Kopecny J., Verdu E., Tlaskalova-Hogenova H.,

Oral administration of Parabacteroides distasonis antigens attenuates experimental murine colitis through

modulation of immunity and microbiota composition.

           Clinical and Experimental Immunology 163 (2), 250 – 259, 2011

Zákostelská Z., Kverka M., Klimešová K., Rossmann P., Mrázek J., Kopečný J., Hornová M., Šrutková D., Hudcovič T., 

          Ridl J., Tlaskalová-Hogenová H.,

          Lysate of probiotic Lactobacillus casei DN-114 001 ameliorates colitis by strengthening the gut barrier function

          and changing the gut microenvironment

           PLOS ONE   6 (11),  e27961,  2011

Bunešová V., Domig K.J., Killer J., Vlková E., Kopečný J., Mrázek J., Ročková Š., Rada V.

            Characterization of bifidobacteria suitable for probiotic use in calves,

Anaerobe 18, 166 – 168, 2012,

Vlčková K., Mrázek J., Kopečný j., Petrželková K.J.

Evaluation of different storage methods to characterize the fecal bacterial communities of captive western

lowland gorillas (Gorilla gorilla gorilla). J. of Microbiological Methods, 91, 45 – 51, 2012

Killer J., Mrázek J., Bunešová V., Havlik J., Koppová I., Benada O., Rada V., Kopečný J.,

            Vlková E.,

            Pseudoscardovia suis gen. nov., sp. nov., a new member of the family Bifidobacteriaceae

isolated from the digestive tract of wild pigs (Sus scrofa). Systematic & Applied Microbiol., 36, 11-16, 2013

Klimešová K., Kverka M., Zákostelecká Z., Hrnčíř T., Hudcovic T., Štěpánková R., Rossman P.,

            Rídl J.,  Kostovčík M., Mrázek J., Kopečný J., Tlaskalová H., 

Altered Gut Microbiota Promotes Colitis-Associated Cancer in IL-1 Receptor–Associated Kinase M–Deficient Mice.

Inflammatory Bowel Diseases,19, (6), 1266 – 1277, 2013

Killer J., Sedláček I., Rada V., Havlík J., Kopečný J.,

            Reclassification of Bifidobacterium stercoris (Kim et al., 2010) as a later heterotypic synonym

            to Bifidobacterium adolescentis.International Journal of Systematic and Evolutionary

            Microbiology, 2013 63 (11): 4350-4353;

Killer J., Ročková S., Bunešová V., Vlková E., Rada V., Kopecny J., Havlik J., Koppová I,

            Benada O., Mrázek J., Kofronová O., Profousová I.,

            Alloscardovia macacae sp. nov., isolated from the milk of macaque (Macaca mulatta),

            emended and revised description of the genus Alloscardovia.   

            International Journal of Systematic and Evolutionary Microbiology, 63 (12), 4439 – 46, 2013

Killer J., Švec P., Sedláček I., Černohlávková J., Benada O., Hroncová Z., Havlík J., Vlková E.,

            Rada V., Kopečný J., Kofroňová O.

            Vagococcus entomophilum sp. nov., from the digestive tract of wasp (Vespula vulgaris)

             International Journal of Systematic and Evolutionary Microbiology 64 (3), 731-737, 2014

Fliegerova K., Tapio I., Bonin A., Mrazek J., Callegari M.L., Bani P., Bayat A., Vilkki J.,

            Kopečný J., Shingfield K.J., Boyer F., Coissac E., Taberlet P., Wallace R.J.

            Effect of DNA extraction and sample preservation method on rumen bacterial population

            Anaerobe, 29, 80 - 84, 2014

Killer J., Havlík J., Vlková E., Rada V., Pechar R., Benada O., Kopečný J., Kofroňová O.,

             Sechovcová H.

            Lactobacillus rodentium sp. nov., from the digestive tract of wild rodents.

            International Journal of Systematic and Evolutionary Microbiology, 64,(5), 1526 - 33, 2014

Henderson G., Cox F., Ganesh S., Jonker A., + consortium

Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range Nature Scientific Reports, 5:14567, 2015                                                                                                       

Rosero J.A., Killer J., Sechovcová H., Mrázek J., Benada O., Fliegerová K., Havlík J., Kopečný J.

            Reclassification of Eubacterium rectale (Prévot et al., 1967) in a new genus Agathobacter gen. nov.,

            as Agathobacter rectalis comb. nov., within the family Lachnospiraceae, and description of

            Agathobacter ruminis sp. nov., from the rumen International Journal of Systematic and Evolutionary Microbiology, 66(2),            768-773, 2016

Grilli D.J., Mrázek J., Fliegerová K., Kopečný J, Lama S.P., Cucchi M.E.C., Sosa M.A.,

            Arenas G.N. Ruminal bacterial community changes during adaptation of goats to fresh alfalfa

            forage. Livestock Science 191, 191–195, 2016

Grilli D.J., Fliegerová K., Kopečný J., Lamac S.P.,  Egea V., Sohaefer N., Pereyra C., Ruiz M. S.,

            Sosa M.A., Arenas G.N., Mrázek J.

             Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet.

            Anaerobe, 42, 17 - 26, 2016

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