Date and place of birth: 15. 1. 1981, Melnik, Czech Republic
Education
| 2005-2009 | Ph.D., developmental biology - Faculty of Science, Charles University, Prague; Doctoral thesis: Cell signaling pathways controlling meiotic maturation of mammalian oocytes |
| 1999-2005 | Faculty of Science, Charles University,Prague, Developmental Biology (MSc.) Diploma thesis: Role of Protein Kinase B during mouse resumption of meiosis |
Professional experience
| 2013-now | Research group leader of Laboratory of DNA integrity, IAPG CAS |
| 2009-2013 | Post-doc, Laboratory of Cell Regeneration and Plasticity, IAPG CAS |
| 2005-now | External teacher, Dept. of Cell Biology, Faculty of Science, Charles University |
International training courses and short stays
| 05/2013 | EMBL Master Course, Bioimage Data Analysis, EMBL Heidelberg, Germany |
| 09/2010 | Department of Biology, University of Pennsylvania, USA, Richard Schultz and Mike Lampson labs, short stay focused on FRET kinase biosensors |
| 11/2007 | European Molecular Biology Laboratory, Heidelberg, Germany, Jan Ellenberg group, short stay, topic: live-cell imaging of oocytes, supported by EMBO short-term fellowship: „CDC25A and CDC25B dynamics during meiotic maturation in the mouse oocytes“ |
| 06/2007 | EMBO practical Course on Quantification of Target Sequences by qPCR, EMBL Heidelberg, Germany |
Teaching Activities
Lectures at Universities
| 2005-now | semester course, Molecular Mechanisms of the Cell Cycle Regulation, Faculty of Science, Charles University, Prague |
| 2017 | selected talks in Reproductive Medicine and Genetics course, Second Faculty of Medicine, Charles University, Prague |
Ph.D. and Diploma thesis supervisions
- Present: supervision of three Ph.D. theses and one diploma thesis.
- Past: supervision of one defended Ph.D. thesis, 2 defended diploma thesis and 2 defended bachelor thesis.
Administrative experience and faculty responsibilities
| 2014-now | Internal member of the Scientific Board, Centre Pigmod |
| 2012-2017 | Vice-head of Council of Inst. of Animal Physiology and Genetics CAS |
Service to scientific community
- Review manuscripts:
- Developmental Dynamics
- Molecular and Cellular Biology
- Cloning and Stem Cells
- Reproduction
- Fertlity and Development
- Plos One
- Ad hoc reviewer of project proposals and theses for Charles University, Prague
- Review of project proposal for Research Councils UK.
International cooperation
- prof. Richard Schultz, University of Pennsylvania and University of California, Davis, USA.
Common topics: Cell cycle regulation in mouse oocytes and embryos
6 collaborative papers, collaborative grant (2012-2015) - Tomoya Kitajima, Ph.D., RIKEN Center for Developmental Biology, Japan
Common topics: Chromosome dynamics and advance live cell imaging techniques
2 collaborative papares - prof. Jan Ellenberg, The European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
Common topics: Live cell imaging of mouse oocytes and early embryos
one collaborative paper - Vladimir Baran, Ph.D., Institute of Animal Physiology, Slovakia
Common topics: Cell signaling in mouse oocytes and embryos, confocal microscopy
9 common papers - Karen Schindler, Ph.D., Department of Genetics, Rutgers, The State University of NJ, USA
Common topic: Chromosome missegregation in infertility
one collaborative paper, collaborative grant under review
Membership in academic councils and editorial boards
| since 2015 | member of The Czechoslovak Microscopy Organization |
| since 2014 | member of the European Society of Human Reproduction and Embryology |
| since 2012 | head of Section of Developmental and Reproduction Biology, Czechoslovak Biological Society |
Grants (PI position)
| 2012-2015 | The signaling pathways synchronizing nuclear envelope break down, chromosome condensation a spindle formation during mammalian meiosis of oocytes, Ministry of Education, program Kontakt II, LH12057 |
| 2010-2013 | Role of CDC25B phosphatase during cell cycle resumption: The comparison of meiotic resumption in oocytes and G2 checkpoint recovery in somatic cells, GACR, P301/11/P081 |
Lectures and seminars (last five years)
| 2016 | Chromosome integrity and dynamics during oocyte meiotic maturation. University of California, Davis, hosted by Richard Schultz, December 8th, 2016 |
| DNA damage response during mouse oocyte maturation. Rutgers University, hosted by Karen Schindler, December 2nd, 2016 | |
| Chromosome integrity and dynamics during oocyte meiotic maturation. Johns Hopkins Medicine, Baltimore, USA, hosted by Rong Li, November 28th, 2016 | |
| 2015 | Multiple requirements of PLK1 during mouse oocyte maturation. Live cell confocal microscopy – new dimension for 21st century. March 11th-13th, 2015, Kosice, Slovakia |
| PLK1 as a key regulator of oocyte meiotic maturation. DNA Analysis XII, June 11th, 2015, 1st Faculty of Medicine, Charles University | |
| 2014 | Multiple requirements of PLK1 during mouse oocyte maturation. Rutgers University, NJ, USA, hosted by Karen Schindler, December 12th, 2014. |
| Multiple requirements of PLK1 during mouse oocyte maturation. University of California, Davis, CA, USA, hosted by Richard Schultz, December 4th, 2014. | |
| 2012 | Check-point kinase I (CHK1) and Polo-like kinase I (PLK1) control multiple aspects of oocytes maturation. Ovarian club II, Prague, November 8th-11th, 2012 |
| Polo-like Kinase I Controls Nuclear Envelope Break Down and Chromosome Dynamics in Meiosis I. SRD-NIAS the 2nd Japan-Czech Joint Symposium “Understanding the Germ Cell Potential”, Tokyo, Japan, September 10th, 2012 |
Publications
Full bibliography in Pubmed collection in total 14 papers in impacted journals including 6 papers with corresponding author position.