RNDr. Lukáš Choleva, Ph.D.


2010 Postdoc
Department of Vertebrate Evolutionary Biology and Genetics, Laboratory of Fish Genetics, IAPG AS CR, v.v.i.
2004-2010 Ph.D.
Department of Vertebrate Evolutionary Biology and Genetics, Laboratory of Fish Genetics, IAPG AS CR, v.v.i.
Department of Zoology, Faculty of Science, Charles University in Prague
Dissertation Thesis: Lost sex: multilocus DNA evolution the hybrid fish complex Cobitis taenia (Teleostei).
Charles University in Prague, Prague 2009.
2008 Dr.
Department of Zoology, Faculty of Science, Charles University in Prague.
2001-2004 Master degree
Faculty of Science, Charles University in Prague
1998-2001 Bachelor degree
Faculty of Science, University of Ostrava


Major scientific interests

We take interest in studies on relationships among phenomena of sexuality, hybridization, clonality (unisexuality) and polyploidy. Here, vertebrate hybrid complexes have been recognised as especially interesting models. We use fishes – spined loaches of Cobitis taenia hybrid complex and amphibians – water frogs of Pelophylax esculentus complex (former Rana esculenta complex) as model systems, as coexistence of sexual species and clonal (spined loaches) or hemiclonal (water frogs) hybrids and polyploidy have all been discovered there.

Currently, we focus on studies on:

  • the origin of unisexuality and polyploidy in natural populations and under experimental crosses in the laboratory
  • the evolutionary history and biogeography of unisexual complexes
  • the dynamics of contemporaty hybrid zones


Fellowships, prizes

2009 The publication of the year 2008 of IAPG AS CR, v.v.i., 1st place (Choleva et al. Philosophical Transactions of the Royal Society B : Biological Sciences 363: 2911-2919)
2009 The publication of the year 2008 of IAPG AS CR, v.v.i., 2nd place (Kotlík, Marková, Choleva et al. Molecular Ecology 17: 1076-1088)


Membership in academic councils and editorial boards

  • Czech Herpetological Society
  • Czech Ornithological Society


Research fellowships and study visits

11/2010, 03/2011 Laboratory of Animal Diversity and Systematics, Katholieke Universiteit Leuven, Belgium
02-03/2006 Evolutionary history of European threatened species and hybrid richness of spiny loaches ( Cobitis, Teleostei ): a multilocus phylogenetic analysis. Scientific stay within the frame of European project SYNTHESYS (Synthesis of systematic resources under European Union) ES-TAF-1691. SYNTHESYS. Prof. I. Doadrio, Biodiversidad i Biologia Evolutiva, MNCN CSIC, Madrid, Spain.
01/2005-12/2007 Phylogeographic relationship of Ponto-Caspian refuge and Central Europe fish populations-sound basis for management and conservation. Joint Research project No. 6 between Czech Academy of Science and Bulgarian Academy of Science. Participant.
10/2007-02/2008 Unprotected hybrid richness: a conservation genetic approach on an example of spined loaches ( Cobitis, Teleostei ). Scientific stay in the frame of European Science Foundation under European Union, Conservation Genetics (CONGEN) Exchange Grant No. 1 384). Prof. I. Doadrio, Biodiversidad i Biologia Evolutiva, MNCN CSIC, Madrid, Spain.



2012 Choleva L., Janko K., De Gelas K., Bohlen J., Šlechtová V., Rábová M. and Ráb P. Synthesis of clonality and polyploidy in vertebrate animals by hybridization between two sexual species. Evolution. Accepted manuscript online.
DOI: 10.1111/j.1558-5646.2012.01589.x
2011 Fijarczyk, A., Nadachowska K., Sebastian S., Babik W., Litvinchuk S., ; Stuglik M., Gollmann, G., Choleva L., Cogălniceanu D., Vukov T., Džukić G., Szymura J. Nuclear and mitochondrial phylogeography of the European fire-bellied toads Bombina bombina and B. variegata supports their independent histories. Molecular Ecology 20: 3381–3398.
DOI: 10.1111/j.1365-294X.2011.05175.x
  Maciak S., Janko K., Kotusz J., Choleva L., Boron´ A., Juchno D., Kujawa R., Kozłowski J., Konarzewski M. Standard metabolic rate is inversely related to erythrocyte and genome size in allopolyploid ?sh of the Cobitis taenia hybrid complex. Functional Ecology 25: 1072-1078.
DOI: 10.1111/j.1365-2435.2011.01870.x
  Říčanová Š., Bryja J., Cosson J.-F., Gedeon C., Choleva L., Ambros A., Sedláček F. Depleted genetic variation of the European ground squirrel in Central Europe in both microsatellites and the major histocompatibility complex gene: implications for conservation. Conservation Genetics 12: 1115-1129.
DOI: 10.1007/s10592-011-0213-1
  Zaleśna A., Choleva, L., Ogielska M., Rábová M. , Marec F., Ráb P.: Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog Pelophylax esculentus by Genomic in situ Hybridization . Cytogenetic and Genome Research 134: 206-212.
DOI: 10.1159/000327716
2008 Choleva, L., Apostolos A., Ráb, P. Janko, K.: Making it on their own: sperm-dependent hybrid loaches switch the sexual hosts and expand beyond the ranges of their original sperm-donors. Philosophical Transactions of the Royal Society B: Biological Sciences 363 (1505): 2911-2919.
DOI: 10.1098/rstb.2008.0059
  Kotlík, P., Marková, S., Choleva, L., Bogutskaya, N.G., Ekmekci, F.G., Ivanova, P.P. Divergence with gene flow between Ponto-Caspian refugia in an anadromous cyprinid Rutilus frisii revealed by multiple gene phylogeography. Molecular Ecology 17: 1076-1088.
DOI: 10.1111/j.1365-294X.2007.03638.x
  Buj, I., Podnar, M., Mrakovčić, M., Choleva, L., Šlechtová, V., Tvrtković, N., Ćaleta, M., Mustafić, P., Marčić, Z., Zanella, D., Brigić, A. Genetic diversity and phylogenetic relationships of spined loaches (genus Cobitis ) in Croatia based on mtDNA and allozyme analyses. Folia Zoologica 57 (1-2): 71-82.
  Šanda, R., Vukić, J., Choleva, L. Křížek, J. Šedivá, A., Shumka, S. Wilson, I.F. Distribution of loach fishes ( Cobitidae , Nemacheilidae) in Albania, with genetic analysis of populations of Cobitis ohridana . Folia Zoologica 57 (1-2): 42-50.
2007 Janko, K., Flajšhans, M., Choleva, L., Bohlen, J., Šlechtová, V., Rábová, M., Lajbner, Z., Šlechta, V., Ivanova. P., Dobrovolov, I., Culling, M., Persat, H., Kotusz, J. & Ráb, P. Diversity of European spined loaches (genus Cobitis L.): an update of the geographic distribution of the Cobitis taenia hybrid complex with a description of new molecular tools for species determination. Journal of Fish Biology 71: 387-408. DOI: 10.1111/j.1095-8649.2007.01663.x