Education 
| 1985-1997 | DSc., Microbiology, Institute of Animal Physiology and Genetics. Microbial Feed degradation in the Rumen. |
| 1977-1981 | CSc., Biochemistry, (eq. Ph.D.), Czechoslovak Academy of Sciences In vitro incubation of rumen microbes. supervisor: Ing. Stanislav Bartoš, DrSc. |
| 1970-1975 | Dipl. Ing., Biochemistry, Institute of Chemical Technology, Prague Stability of lysosomes in meat post-mortem. supervisor: prof. Ing. Jan Káš, DrSc. |
Professional experience
| 2017-now | Deputy director for Science |
| 2007-2016 | Director of IAPG CAS, v. v. i. |
| 2003 | Head of Laboratory of Anaerobic Microbiology |
| 1991 | Senior Scientific Officer |
Major scientific interests
- Anaerobic Microbiology
My studies started with rumen proteolytic, amylolytic and cellulolytic microbes and their hydrolytic enzyme production. Following years I was dealing with rumen microbes adhesion to feed particles. We introduced methods for anaerobic microbiology and developed probiotics for in vivo testing. Then we moved to biochemistry of rumen bacterium Butyrivibrio fibrisolvens and rumen fungi and interactions of rumen microbes in vitro and in vivo. It led to the development of enzymatic method for estimation of protein degradation in the rumen and feed digestibility for ruminants. I took a part in transformation of anaerobes. I was able with a shuttle vector with fluoroacetate dehalogenase to transform a many Pseudobutyrivibrio isolates. Transformation of undescribed isolates directed me to taxonomy of butyrate-producing bacteria and description of two new species: Butyrivibrio hungatei, Pseudobutyrivibrio xylanivorans. I was involved in description of Butyrivibrio proteoclasticus, later on.
Knowledge of butyrate-producing bacteria helped me to map changed microbiome in patients with the human bowel diseases: we described the effect of Inflammation Bowel Disease and Celiac Disease and we able to show the importance of probiotic treatment. It was possible to describe Bifidobacteria gut colonization in newborns, calves as well as in social insect digestive tract.
In last decade, we have detected presence of bifidobacteria in most vertebrates and many insect species. In frame of this, we described new bifidobacteria in the gut of bumblebees: Bifidobacterium bombi, B. bohemicum and B. actinicoloniformis and a new genus Bombiscardovia (Bombiscardovia coagulans).
Membership in academic councils and editorial boards
| 2012 | Journal of Nutritional Ecology and Food Research, Editorial Board |
| 2010 | Folia Microbiologica, Editorial Board |
| 2009 | Bioprospect, Editorial Board |
| 2009 | Czech Society for Immunology |
| 1999 | Biotechnology Society of CR |
| 1985 | Czechoslovak Society for Microbiology |
| 1983 | Czech Society for biochemistry and Molecular biology |
| 1981-2003 | American Society for Microbiology |
Grants
| 2016-2018 | Kopečný J. Structure and synergism of fibrolytic enzymes in the rumen. Czech Science Foundation, 16-12431S |
| 2015-2019 | Němečková Irena, MILCOM, Kopečný J. Fermented dairy products and cheeses for healthy human nutrition, technological procedures of their production and evaluation methods accenting high level of microbiological safety and improved nutritional parameters. NAZV QJ1510338 |
| 2012-2015 | Wallace R. John, Rowett (UK), Kopečný J. Ruminomics: Connecting the animal genome, gastrointestinal microbiomes and nutrition to improve digestion efficiency and the environmental impacts of ruminant livestock production. FP7 289319 |
| 2011-2014 | Tlaskalová Helena, MBÚ, Kopečný J. Bacteria in etiology, prevention and therapy of experimentally induced intestinal inflammation and colorectal carcinoma. GA ČR 304/11/1252. |
Publications
| 2016 | Grilli D.J., Fliegerová K., Kopečný J., Lamac S.P., Egea V., Sohaefer N., Pereyra C., Ruiz M. S., Sosa M.A., Arenas G.N., Mrázek J. Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet. Anaerobe, 42, 17 - 26 |
| Grilli D.J., Mrázek J., Fliegerová K., Kopečný J, Lama S.P., Cucchi M.E.C., Sosa M.A., Arenas G.N. Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage. Livestock Science 191, 191–195, 2016 |
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| Rosero J.A., Killer J., Sechovcová H., Mrázek J., Benada O., Fliegerová K., Havlík J., Kopečný J. Reclassification of Eubacterium rectale (Prévot et al., 1967) in a new genus Agathobacter gen. nov., as Agathobacter rectalis comb. nov., within the family Lachnospiraceae, and description of Agathobacter ruminis sp. nov., from the rumen International Journal of Systematic and Evolutionary Microbiology, 66(2), 768-773, 2016 |
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| 2014 | Killer J., Švec P., Sedláček I., Černohlávková J., Benada O., Hroncová Z., Havlík J., Vlková E., Rada V., Kopečný J., Kofroňová O. Vagococcus entomophilum sp. nov., from the digestive tract of wasp (Vespula vulgaris) International Journal of Systematic and Evolutionary Microbiology 64 (3), 731-737, 2014 |
| Fliegerova K., Tapio I., Bonin A., Mrazek J., Callegari M.L., Bani P., Bayat A., Vilkki J., Kopečný J., Shingfield K.J., Boyer F., Coissac E., Taberlet P., Wallace R.J. Effect of DNA extraction and sample preservation method on rumen bacterial population Anaerobe, 29, 80 - 84, 2014 |
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| Killer J., Havlík J., Vlková E., Rada V., Pechar R., Benada O., Kopečný J., Kofroňová O., Sechovcová H. Lactobacillus rodentium sp. nov., from the digestive tract of wild rodents. International Journal of Systematic and Evolutionary Microbiology, 64,(5), 1526 - 33, 2014 |
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| 2013 | Killer J., Mrázek J., Bunešová V., Havlik J., Koppová I., Benada O., Rada V., Kopečný J., Vlková E., Pseudoscardovia suis gen. nov., sp. nov., a new member of the family Bifidobacteriaceae isolated from the digestive tract of wild pigs (Sus scrofa). Systematic & Applied Microbiol., 36, 11-16, 2013 |
| Klimešová K., Kverka M., Zákostelecká Z., Hrnčíř T., Hudcovic T., Štěpánková R., Rossman P., Rídl J., Kostovčík M., Mrázek J., Kopečný J., Tlaskalová H., Altered Gut Microbiota Promotes Colitis-Associated Cancer in IL-1 Receptor–Associated Kinase M–Deficient Mice. Inflammatory Bowel Diseases,19, (6), 1266 – 1277, 2013 |
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| Killer J., Sedláček I., Rada V., Havlík J., Kopečný J., Reclassification of Bifidobacterium stercoris (Kim et al., 2010) as a later heterotypic synonym to Bifidobacterium adolescentis.International Journal of Systematic and Evolutionary Microbiology, 2013 63 (11): 4350-4353; |
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| Killer J., Ročková S., Bunešová V., Vlková E., Rada V., Kopecny J., Havlik J., Koppová I, Benada O., Mrázek J., Kofronová O., Profousová I., Alloscardovia macacae sp. nov., isolated from the milk of macaque (Macaca mulatta), emended and revised description of the genus Alloscardovia. International Journal of Systematic and Evolutionary Microbiology, 63 (12), 4439 – 46, 2013 |
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| 2012 | Bunešová V., Domig K.J., Killer J., Vlková E., Kopečný J., Mrázek J., Ročková Š., Rada V. Characterization of bifidobacteria suitable for probiotic use in calves, Anaerobe 18, 166 – 168, 2012 |
| Vlčková K., Mrázek J., Kopečný j., Petrželková K.J. Evaluation of different storage methods to characterize the fecal bacterial communities of captive western lowland gorillas (Gorilla gorilla gorilla). J. of Microbiological Methods, 91, 45 – 51, 2012 |
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| 2011 | Zákostelská Z., Kverka M., Klimešová K., Rossmann P., Mrázek J., Kopečný J., Hornová M., Šrutková D., Hudcovič T., Ridl J., Tlaskalová-Hogenová H., Lysate of probiotic Lactobacillus casei DN-114 001 ameliorates colitis by strengthening the gut barrier function and changing the gut microenvironment PLOS ONE 6 (11), e27961, 2011 |
| Kverka M., Zakostelska Z., Klimesova K., Sokol D., Hudcovic T., Hrncir T., Rossmann P., Mrazek J., Kopecny J., Verdu E., Tlaskalova-Hogenova H., Oral administration of Parabacteroides distasonis antigens attenuates experimental murine colitis through modulation of immunity and microbiota composition. Clinical and Experimental Immunology 163 (2), 250 – 259, 2011 |