Mouse primary non-cancerous cell expressing H2B-mCHERRY (red) and PCNA-GFP (green) from transfected recombinant mRNA. Punctuated green signal marks progression through S-phase (DNA replication). Note for defective chromosome segregation in mitosis.
Mouse oocyte expressing H2B-mCHERRY (red) and MAP4-GFP (green) after mRNA microinjection progresses through meiotic maturation. Spindle formation, chromosome segregation and 1st polar body extrusion are visible.
Mouse oocyte from transgenic CAG::H2B-EGFP mouse stained with fluorogenic compound SiR tubulin for microtubule visualization. Chromosomes (H2B) are pseudocolored by red, microtubules are green. In contrast to Movie 2, oocyte was only vitally stained by SiR tubulin without any mRNA microinjection.
Defective chromosome segregation (red) in oocyte after induction of double strand DNA breaks using radiomimetic drug Neocarcinostation (NCS) in prophase I (see also Movie 5). Oocyte is from transgenic CAG::H2B-EGFP mouse strain.
3D projection of the nucleus of fixed mouse oocytes in prophase I after induction of double strand DNA breaks (DSBs) using radiomimetic drug Neocarcinostatin (NCS). DNA is stained by DAPI (blue), DSBs are visible as yellow dots for localization of DSB markers gH2AX (green) and MDC1 (red) to the site of DSB.